Determination of the hematocrit value by centrifugation

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Determination of the hematocrit value

The hematocrit value indicates the volumetric proportion of red blood cells in the blood.

Centrifugation is the reference method for the determination of this value. It is used to separate the cellular components of the blood from the fluid ones and to package them tightly together. The reference method is described in DIN 58933-1. According to it, glass capillaries are centrifuged until the product of the minimum relative centrifugal force (RCF > 5000) acting on the erythrocytes and the numerical value for the centrifuging time in minutes is at least 100 000.

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