Slide preparations for urine cytology Preparation of native urine

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Slide preparations for urine cytology

To derive a good cytological preparation from a urine sample it is not sufficient to simply centrifuge native urine onto commercial microscope slides. Urine has a low cell concentration and those cells present adhere poorly to the slides. This would result in a preparation with an insufficient cell concentration. To increase the cell concentration it is necessary to coat the slide or use a suitable fixative. Poly-L-Lysine (PLL, Sigma, Cat. No. P8920) has been found to be suitable for slide coating and Saccomano's solution for fixing.

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